Review





Similar Products

nonspecific antibody binding  (Boster Bio)
94
Boster Bio nonspecific antibody binding
Nonspecific Antibody Binding, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pmc13021461-181-2-8?v=Boster+Bio
Average 94 stars, based on 1 article reviews
nonspecific antibody binding - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
nonspecific igg binding  (Vector Laboratories)
96
Vector Laboratories nonspecific igg binding
Nonspecific Igg Binding, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pmc12310649-164-0-10?v=Vector+Laboratories
Average 96 stars, based on 1 article reviews
nonspecific igg binding - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
nonspecific antibody binding  (Baxter Healthcare)
86
Baxter Healthcare nonspecific antibody binding
Nonspecific Antibody Binding, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/10__1111_slash_1756___185x__70292-60-21-10?v=Baxter+Healthcare
Average 86 stars, based on 1 article reviews
nonspecific antibody binding - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
nonspecific antibody binding  (Vector Laboratories)
96
Vector Laboratories nonspecific antibody binding
Nonspecific Antibody Binding, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pm40148437-248-21-32?v=Vector+Laboratories
Average 96 stars, based on 1 article reviews
nonspecific antibody binding - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
nonspecific antigen binding  (Novus Biologicals)
95
Novus Biologicals nonspecific antigen binding
Nonspecific Antigen Binding, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pm39832583-195-9-17?v=Novus+Biologicals
Average 95 stars, based on 1 article reviews
nonspecific antigen binding - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
nonspecific protein binding sites  (Proteintech)
96
Proteintech nonspecific protein binding sites
Nonspecific Protein Binding Sites, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pm39688535-237-22-34?v=Proteintech
Average 96 stars, based on 1 article reviews
nonspecific protein binding sites - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
nonspecific antibody binding tubes (nabt, catalog number 3ix761)  (Scantibodies inc)
90
Scantibodies inc nonspecific antibody binding tubes (nabt, catalog number 3ix761)
Nonspecific Antibody Binding Tubes (Nabt, Catalog Number 3ix761), supplied by Scantibodies inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pm39396631-69-15-23?v=Scantibodies+inc
Average 90 stars, based on 1 article reviews
nonspecific antibody binding tubes (nabt, catalog number 3ix761) - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
nonspecific binding  (Bio X Cell)
96
Bio X Cell nonspecific binding
Figure 9. CD8+ T cell depletion ameliorates psoriasis and arthritis in humanized mice. (A) PsA mice were injected with anti-CD8 or isotype control anti- bodies at the time of PBMC injection. At day 30 after transfer, mouse PBMCs were stained with a DEAD/LIVE marker and <t>nonspecific</t> binding blocked with human TruStain FcX Fc receptor blocking solution. The percentage of CD19–CD3+CD8+ T cells was defined with FlowJo to determine the efficiency of CD8+ T cell depletion. (B and C) hu-PsA mice injected with CD8 antibodies did not develop psoriasiform lesions or ankle swelling, compared with mice injected with isotype control antibodies. (D) Epidermal hypertrophy and numerous dermal infiltrating cells were observed in the skin of hu-PsA mice injected with isotype control antibodies. (E) Infiltrating CD3+ T cells (red) and Ki67+ proliferating cells in skin of humanized mice. (F) CD8+ T cell depletion significantly reduced epidermal thickness in hu-PsA mice. (G) Pannus areas were visualized in hu-PsA treated with isotype control or CD8+ T cell–depleting antibodies. The dashed line outlines pannus areas. (H) Proliferative type 1 T cells (CD3, red; T-bet, green; Ki67, white) and proliferating T cells (CD3, red; Ki67, white) were decreased in CD8+ T cell–depleted mice. Representative images of Alcian Blue and H&E stain were taken with an Olympus VS120 scanner. Scale bars: 1,000 μm. IF 3 × 3 mosaic images were taken (original magnification, ×200) with a Zeiss Axioplan microscope and recorded with a Hamamatsu camera. (I) Quantitation of CD3+ T cells, CD3+Ki67+ proliferating T cells, and CD3+T-bet+ type 1 T cells in ankle synovial tissue of hu-PsA mice. Scale bar: 200 μm. n = 5 mice reconstituted with PBMCs and sera from different patients with PsA in each group. Significance was calculated with 2-way ANOVA and Tukey’s multiple comparison test. *P ≤ 0.05; **P ≤ 0.005; ****P ≤ 0.0001.
Nonspecific Binding, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pm39114979-268-0-15?v=Bio+X+Cell
Average 96 stars, based on 1 article reviews
nonspecific binding - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
nonspecific binding sites  (Proteintech)
96
Proteintech nonspecific binding sites
Figure 9. CD8+ T cell depletion ameliorates psoriasis and arthritis in humanized mice. (A) PsA mice were injected with anti-CD8 or isotype control anti- bodies at the time of PBMC injection. At day 30 after transfer, mouse PBMCs were stained with a DEAD/LIVE marker and <t>nonspecific</t> binding blocked with human TruStain FcX Fc receptor blocking solution. The percentage of CD19–CD3+CD8+ T cells was defined with FlowJo to determine the efficiency of CD8+ T cell depletion. (B and C) hu-PsA mice injected with CD8 antibodies did not develop psoriasiform lesions or ankle swelling, compared with mice injected with isotype control antibodies. (D) Epidermal hypertrophy and numerous dermal infiltrating cells were observed in the skin of hu-PsA mice injected with isotype control antibodies. (E) Infiltrating CD3+ T cells (red) and Ki67+ proliferating cells in skin of humanized mice. (F) CD8+ T cell depletion significantly reduced epidermal thickness in hu-PsA mice. (G) Pannus areas were visualized in hu-PsA treated with isotype control or CD8+ T cell–depleting antibodies. The dashed line outlines pannus areas. (H) Proliferative type 1 T cells (CD3, red; T-bet, green; Ki67, white) and proliferating T cells (CD3, red; Ki67, white) were decreased in CD8+ T cell–depleted mice. Representative images of Alcian Blue and H&E stain were taken with an Olympus VS120 scanner. Scale bars: 1,000 μm. IF 3 × 3 mosaic images were taken (original magnification, ×200) with a Zeiss Axioplan microscope and recorded with a Hamamatsu camera. (I) Quantitation of CD3+ T cells, CD3+Ki67+ proliferating T cells, and CD3+T-bet+ type 1 T cells in ankle synovial tissue of hu-PsA mice. Scale bar: 200 μm. n = 5 mice reconstituted with PBMCs and sera from different patients with PsA in each group. Significance was calculated with 2-way ANOVA and Tukey’s multiple comparison test. *P ≤ 0.05; **P ≤ 0.005; ****P ≤ 0.0001.
Nonspecific Binding Sites, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonspecific+antibody+binding/pm38919032-819-28-37?v=Proteintech
Average 96 stars, based on 1 article reviews
nonspecific binding sites - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier

Image Search Results


Figure 9. CD8+ T cell depletion ameliorates psoriasis and arthritis in humanized mice. (A) PsA mice were injected with anti-CD8 or isotype control anti- bodies at the time of PBMC injection. At day 30 after transfer, mouse PBMCs were stained with a DEAD/LIVE marker and nonspecific binding blocked with human TruStain FcX Fc receptor blocking solution. The percentage of CD19–CD3+CD8+ T cells was defined with FlowJo to determine the efficiency of CD8+ T cell depletion. (B and C) hu-PsA mice injected with CD8 antibodies did not develop psoriasiform lesions or ankle swelling, compared with mice injected with isotype control antibodies. (D) Epidermal hypertrophy and numerous dermal infiltrating cells were observed in the skin of hu-PsA mice injected with isotype control antibodies. (E) Infiltrating CD3+ T cells (red) and Ki67+ proliferating cells in skin of humanized mice. (F) CD8+ T cell depletion significantly reduced epidermal thickness in hu-PsA mice. (G) Pannus areas were visualized in hu-PsA treated with isotype control or CD8+ T cell–depleting antibodies. The dashed line outlines pannus areas. (H) Proliferative type 1 T cells (CD3, red; T-bet, green; Ki67, white) and proliferating T cells (CD3, red; Ki67, white) were decreased in CD8+ T cell–depleted mice. Representative images of Alcian Blue and H&E stain were taken with an Olympus VS120 scanner. Scale bars: 1,000 μm. IF 3 × 3 mosaic images were taken (original magnification, ×200) with a Zeiss Axioplan microscope and recorded with a Hamamatsu camera. (I) Quantitation of CD3+ T cells, CD3+Ki67+ proliferating T cells, and CD3+T-bet+ type 1 T cells in ankle synovial tissue of hu-PsA mice. Scale bar: 200 μm. n = 5 mice reconstituted with PBMCs and sera from different patients with PsA in each group. Significance was calculated with 2-way ANOVA and Tukey’s multiple comparison test. *P ≤ 0.05; **P ≤ 0.005; ****P ≤ 0.0001.

Journal: JCI insight

Article Title: Psoriatic arthritis subtypes are phenocopied in humanized mice.

doi: 10.1172/jci.insight.178213

Figure Lengend Snippet: Figure 9. CD8+ T cell depletion ameliorates psoriasis and arthritis in humanized mice. (A) PsA mice were injected with anti-CD8 or isotype control anti- bodies at the time of PBMC injection. At day 30 after transfer, mouse PBMCs were stained with a DEAD/LIVE marker and nonspecific binding blocked with human TruStain FcX Fc receptor blocking solution. The percentage of CD19–CD3+CD8+ T cells was defined with FlowJo to determine the efficiency of CD8+ T cell depletion. (B and C) hu-PsA mice injected with CD8 antibodies did not develop psoriasiform lesions or ankle swelling, compared with mice injected with isotype control antibodies. (D) Epidermal hypertrophy and numerous dermal infiltrating cells were observed in the skin of hu-PsA mice injected with isotype control antibodies. (E) Infiltrating CD3+ T cells (red) and Ki67+ proliferating cells in skin of humanized mice. (F) CD8+ T cell depletion significantly reduced epidermal thickness in hu-PsA mice. (G) Pannus areas were visualized in hu-PsA treated with isotype control or CD8+ T cell–depleting antibodies. The dashed line outlines pannus areas. (H) Proliferative type 1 T cells (CD3, red; T-bet, green; Ki67, white) and proliferating T cells (CD3, red; Ki67, white) were decreased in CD8+ T cell–depleted mice. Representative images of Alcian Blue and H&E stain were taken with an Olympus VS120 scanner. Scale bars: 1,000 μm. IF 3 × 3 mosaic images were taken (original magnification, ×200) with a Zeiss Axioplan microscope and recorded with a Hamamatsu camera. (I) Quantitation of CD3+ T cells, CD3+Ki67+ proliferating T cells, and CD3+T-bet+ type 1 T cells in ankle synovial tissue of hu-PsA mice. Scale bar: 200 μm. n = 5 mice reconstituted with PBMCs and sera from different patients with PsA in each group. Significance was calculated with 2-way ANOVA and Tukey’s multiple comparison test. *P ≤ 0.05; **P ≤ 0.005; ****P ≤ 0.0001.

Article Snippet: Nonspecific binding was prevented by incubation with 10 μg/mL Fc block (clone 2.4G2, BE0307, RRID:AB_2736987, BioXcell) for 10 minutes on ice.

Techniques: Injection, Control, Staining, Marker, Binding Assay, Blocking Assay, Microscopy, Quantitation Assay, Comparison